Vysis alk break apart fish probe kit abbott molecular. The vysis alk break apart fish probe kit is a qualitative test to detect rearrangements involving the alk gene via fluorescence in situ hybridization fish in formalinfixed paraffinembedded ffpe nonsmall cell lung cancer nsclc tissue specimens to aid in identifying those patients eligible for treatment with xalkori crizotinib. An orange labeled probe hybridizes proximal to cbfb, and a green labeled probe hybridizes distal to cbfb in 16q22. Vysis alk break apart fish probe kit en food and drug.
Cancer is one of the major causes of mortality in ecuador and annually, hematological malignancies are within the top ten most common cancers. Reliably detect rna sequences with novocastra ish probes, detection systems, and ancillary reagents. Mll break apart dnafish probe two color, break apart probe h11002 vc s20ocwf standard fish procedure note. Given the frequency of lowgrade bcell lymphomas other than lpl associated with t9. Igh break apart dnafish probe two color, break apart probe h14 vc s20ocwf standard fish procedure note. The vysis alk break apart fish probe kit uses fluorescence in situ allowed to hybridize with the dna probes. The patient was diagnosed with aml, and treated with arac and idarubicin. If the test is interpreted by a cytotechnologist, procedure codes 88271 and 88275 are applicable. Kreatech fish probes are the latest in advancements in fish probes. Vysis cbfb break apart fish probe kit molecular catalog. Zytolight spec ptencen 10 dual color probe zytovision gmbh. In addition to revealing breaks, which can lead to translocation of parts of the gene, inversion, or its fusion to other genes, the probe set can also be used to identify other tfeb aberrations such as deletions or. Vysis alk break apart fish probe kit ce ivd 20 assay.
Do not reconstitute or dilute with hybridization buffer. Hybridization of the bm smear slide with the vysis cbfb breakapart fish probe kit abbott laboratories, abbott park, il, u. Vysis cep 8 spectrumorange direct labeled fluorescent dna. Exploring the genomewide relation between copy number.
Braf break apart fish probe kit introduction the braf break apart fish probe kit is designed to detect rearrangements in the human braf gene located on chromosome band 7q34. Miniaturized fish for screening of oncohematological. Fish analysis of the touch preparation from the tumour biopsy, using the vysis cbfb break apart probe, detected an abnormal signal pattern with one intact and one split cbfb. Vysis alk break apart fish probe kit abbott molecular contact. Kreatech fish probes are the latest advancement in dna in situ hybridization. Dna for probe use is labeled with fluorescent molecules. Vysis alk break apart fish probe kit hybridization conditions may be adversely affected by the use of 1. Shipping conditions the vysis cll fish probe kit is shipped on dry ice. Although this translocation involving the paired homeobox5 pax5 gene at chromosome band 9p and the immunoglobulin heavy chain igh gene at 14q32 has been described in. An example of application of fish technique in order to specify the nature of the 11q23mll rearrangement. The vysis lsi cbfb dual color break apart rearrangement fluorescence in situ hybridization fish probe is intended to detect chromosomal rearrangements.
The vysis alk break apart fish probe kit is shipped on dry ice. Pdf rearrangement of 11q23 chromosome region in acute. Detection of a novel cbfbmyh11 variant fusion transcript. The 8q24 myc rearrangement is detected using the vysis lsimyc dual color breakapart rearrangement probe kit abbott laboratories, des plaines, il usa. Agilent expands catalog of surefish breakapart probes. Mll break apart dnafish probe two color, break apart. Materials provided if you receive reagents that are in a condition contrary to label vysis alk break apart fish probe kit list no. Spectrumgreen direct labeled fluorescent dna probe kit abbott molecular. A fish test that detects deletion of the lsi tp53, lsi atm, and lsi ds319 probe targets and gain of the d12z3 sequence. Lsi tcf3pbx1 dual color, dual fu sion translocation probe. Analytical and performance characteristics are not established. Developed with the use of repeatfree technology, kreatech fish probes eliminate the use of cot1 or blocking dna, providing a clearer background and a brighter signal novocastra ish probes. Blog archives venetoclax venclexta for the treatment of patients with chronic lymphocytic leukemia cll. Vysis lsi mll dual color, break apart rearrangement probe.
The vysis alk break apart fish probe kit is a qualitative. Vysis alk break apart fish probes kit vysis cll fish probe kit vysis egr1 fish probes kit. Vysis lsi etv6tel runx1aml1 es dual color translocation probe set. Cbfb probes, produced red and green breakapart signals, and a redgreen yellow fusion signal in the nuclei figure 1a. The probes are constructed using the repeatfree technology that is based on subtractive hybridization, which specifically removes all repetitive elements from the probe. Diagnostics safety data sheets sds united states and international. Vysis fus break apart fish probe kit 3n5820 vysis ss18 break apart fish probe kit previously. Vysis cbfbbreak apart probe revealed two intact cbfbsignals in all 100 nuclei examined, thereby excluding an underlying 16q22 clonal rearrangement. Alk iqfish breakapart probe dako omnis 2 warnings and precautions 1 for in vitro diagnostic use. The probes bind to a specific region or regions on the target chromosome. Xl cbfbmyh11 plus is designed as a dual fusion probe. Single color probes are designed to cover a gene of interest. The tumor suppressor gene pten phosphatase and tensin homolog deleted on. Fees reflect 2020 cms medicare fee schedules codemap.
This kit contains a mixture of lsimyc spectrumorange probe and lsimyc spectrumgreen probe. Asr analyte specific reagent analytical and performance characteristics are not established gpr general purpose reagent ivd in vitro diagnostics rx only ruo research use only. Vysis fish probes for solid tumors ilex medical ltd. The xl cbfb probe is designed as a break apart probe with two probes juxtaposed and differently labeled. Please be aware that the website you have requested is intended for the residents of a particular country or region, as noted on that site. Each probe is sold in 20 test kits 20 slides 22x22 mm area and includes hybridization buffer. Empire genomics bcor fish probe is used to detect translocations of the bcor gene and can be labeled in one of five colors, using standard nick translation protocols. Cbfb breakapart probes vysis and kreatech figure 3. Myeloid sarcoma of the small bowel associated with a cbf.
Abl2 break apart fish probe the abl2 break apart fish probe is designed to detect abl2 rearrangements. Fluorescent in situ hybridization fish fish is the detection of highly specific dna probes which have been hybridized to either interphase or metaphase chromosomes using fluorescence microscopy. Igh break apart dnafish probe two color, break apart. Scientists have used 3d models to break down the dna behavior of cancer cells, in a breakthrough new study which could revolutionize treatment for the disease. A fish test that detects rearrangements involving the alk gene in nonsmall cell lung cancer tissue specimens. Vysis lsi cbfb dual color, break apart rearrangement probe 20ul.
The tfeb break apart fish probe kit is designed to detect rearrangements in the human tfeb gene located on chromosome band 6p21. View vysis fish probe maps, hybridization images, and product ordering information for chromosome 16. As a result of miniaturization, fish can be performed with at least a 10fold reduction in probe usage and minimal cell requirements, creating. All metasystems human fish probe products are classified as ivd products in the eu. Calr card11 c11orf30casp8 cbfb cbl ccnd1ccnd2 ccnd3cd22cd274 pdl1 cd70ccne1 cd79a cd79b cdc73 cdh1 cdk12cdk4cdk6 cdk8. Vysis lsi cbfb dual color, break apart rearrangement probe. Fluorescence in situ hybridization fish represents a major step in the analysis of. Cep 8 spectrumorange direct labeled fluorescent dna probe kit without control slides. The xl cll probe kit is a combination of probes covering the most frequent.
The karyotype was further supported by use of the chromoprobe multiprobe system, octachrome whole. In addition to revealing breaks, which can lead to translocation of parts of the gene, inversion, or its fusion to other genes, the probe set can also be used to identify other plag1 aberrations such as deletions or. Fish break apart assays are valuable tools for the detection of ig light chains. Vysis lsi bcrabl es dual color translocation probe t9. Vysis lsi etv6telrunx1aml1 es dual color translocation probe set, vysis cdkn2acep 9 fish probe kit, vysis lsi bcrabl dual color, single fusion translocation probe, vysis lsi tp53 17p.
The plag1 break apart fish probe kit is designed to detect rearrangements in the human plag1 gene mapping to chromosome band 8q12. Search for almost any human gene and chances are we can. The np40 and 20x ssc salt may be stored separately at room temperature. In this multicentric study, we analyzed a series of patients diagnosed with different hematological disorders between the years 1984 and 2012. Vysis fish probes for solid tumors products are analyte specific reagents. Probe map details based on ucsc genome browser grch37hg19. Fish signals from pmlrara, aml1eto, bcrabl, and mll probes abbott molecularvysis, des plaines, il were within reference ranges, whereas the cbfbmyh11 fish showed abnormal break apart signals one green, one orange, and one fusion. The vysis fish chromosome search tool provides the most uptodate vysis fish probe information. Vysis lsi cbfb break apart rearrangement probe asr. We developed a dualcolor breakapart bacterial artificial chromosome bac probe spanning all previously sequenced pax5 rearrangements figure 1. The twocolor probe applied on the break point in the mll gene consists of two parts. Vysis lsi alk dual color break apart fish probe reagents other than those provided by abbott molecular.
You are about to exit for another abbott country or region specific website. Only a technologist familiar with cytogenetic methods and trained for the fish technique can perform the assay. Fivemicron unstained ffpe sections were used for fish analysis. Validation of breakapart and fusion myc probes using a. This patent pending, interactive tool provides singleclick access from fish probe descriptions directly to fish probe maps, hybridization images, and associated product page and ordering information.